Remi LamBI 212 LABJanuary 13, 2018Take Home #1The questions that are investigated in this article are the effects that temperature has on photosynthesis and plant growth, ribulose-phosphate carboxylase (aka Ribisco) content and whole plant growth in the assimilation shoots of a rose (Ayuko Ushio, Tadahiko Mae and Amane Makino 2008). In other words, the purpose of this study is to test how the temperature will affect the photosynthesis and plant growth in the shoots of a rose. The previous work, or theories that were informed from previous studies include “shoot-bending” or “arching” where cultivation techniques resulted in higher yield and better quality of flowering shoot (Okawa and Suematsu 1999). Shoot-bending can be described as basal shoots that emerge during the early stage of growth are artificially bent down to effectively catch the sunlight so that new shoots that emerge successfully are harvested for rose products (Okawa and Suematsu 1999). There were several ways this experiment was approached. One was to lift up the leaf area index also known as LAI of the bent-shoot canopies. The other approach was to enhance the photosynthetic capacity of the absorption of the shoots before shoot bending and to keep a high photosynthetic capacity in the shoots before bending. Since roses are generally cultivated in environments such as a greenhouse, the temperature significantly changes through the year and the difference in temperature between the winter and summer is about 10 degrees Celsius. That is why this previous study differs from present studies because now, experts study and grow the assimilation shoots throughout two different times of the day for 42 days after 1 month of growth. At night, where the temperature is around 30 and 25 degrees Celsius, and during the day where the temperature is approximately 20 and 15 degrees Celsius. Then, proceed to analyze the growth rates of the shoots under these different temperatures. In the illustration above, it shows the rose shoots being tested in different times through the day. A very simple illustration showing the shoot resting under the sun during the day, and at night under the moon. Both giving off very different temperatures. Some contexts of the study that may have affected the results was Rubisco, which is one of the several different photosynthetic enzymes in the plants. They found that it limited the light-saturated photosynthesis under the carbon dioxide levels (Evans 1986; Makino et al. 1985). Therefore, when there was an increase in Rubisco content throughout the growth at lower temperature, it did not lead to the increase in photosynthesis. However, the cause of this miscalculation is still unknown. This experimental design measures the correct variables. Their question stated if temperature affects photosynthesis and plant growth in the assimilation shoots of a rose, and they found significant results. They concluded that the photosynthetic capacity in roses heavily depends on growth temperature even when the nutrition conditions are the same. Their control factor was the weather, because it remained constant throughout the experiment. They came to the conclusion that the rose assimilation shoots are better cultivated at 20/15 degrees Celsius than cultivation at 30/25 degrees Celsius. The results are heavily significant. The results showed that the photosynthetic rate was higher in plants grown in 30/25 degree Celsius during the first seven days. Between the tenth and eighteenth day the results did not show significant differences. On day 42, the photosynthetic rate showed to be higher in the plants grown in the 20/15 degree Celsius environment than the 30/25 degree Celsius plants. All the restrictions increased in 20/15 degree Celsius plants, and decreased in the 30/24 degree Celsius plants after day 14. The conclusion that temperature heavily affects growth rates in assimilation shoots are well supported by the pattern of the results provided. For example, the relative growth rate, net assimilation rate, and leaf area ratio rate are well supported. The only conclusions of the experiment that do not appear to be well supported are the leaf weight ratio and specific leaf area, because they were less accurate results and it became a bit inconsistent. The issue is addressed in this paper of concern to humans because this experiment has potential to lead to other plant based foods. For example, there have been trends where multiple cold habitat plants like spinach (Holaday et al. 1992), Arabidopsis (Strand et al. 1999), winter rye (Hurry et al. 1995), winter wheat and winter rape (Hurry et al. 1995), that grow in low temperatures increases the different photosynthetic enzymes embedded in them. There were no questions provided by the author that suggests further or future research. One question I would ask, however, would be if this experiment would work with the assimilation of corn shoots versus the shoot of a rose.